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玉米秸秆腐解液酚酸物质含量检测及对玉米大斑病菌的影响
Detection of phenolic acids in crop straw decomposed liquid and their effect on pathogen of northern leaf blight of corn
投稿时间:2015-11-13  最后修改时间:2016-03-10
中文关键词:秸秆还田  玉米秸秆腐解液 酚酸物质  玉米大斑病 Exserohilum turcicum
英文关键词:straw returned  crop straw decomposed liquid  phenolic compounds  northern leaf blight of corn  Exserohilum turcicum
基金项目:国家自然科学“双株定向栽培模式对玉米纹枯病流行影响及灾变机制研究”(31171791);国家“粮丰工程”项目“东北平原南部(辽宁)春玉米水稻持续丰产高效技术集成创新与示范课题”(2011BAD16B12);国家“粮丰工程”项目“辽宁春玉米粳稻大面积均衡增产技术集成研究与示范”(2012BAD04B03)作者:郭晓源,男,硕士研究生,研究方向为植物病害流行学,E-mail:guoxiaoyuan9004@163.com*通讯作者:傅俊范,教授,博士生导师,E-mail:fujunfan@163.com 郭晓源 景殿玺 周如军 傅俊范*
作者单位E-mail
郭晓源 沈阳农业大学植物保护学院 guoxiaoyuan9004@163.com 
景殿玺 沈阳农业大学植物保护学院  
周如军 沈阳农业大学植物保护学院  
傅俊范 沈阳农业大学植物保护学院  
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中文摘要:
      近年来,玉米秸秆还田已成为农业生产中常见的耕作方式。本文通过提取不同时期玉米秸秆腐解液,研究其对玉米大斑病菌丝生长和孢子萌发的影响。利用HPLC检测腐解60d的腐解液,推测其含有对羟基苯甲酸、邻苯二甲酸、香草酸、丁香酸和苯甲酸。通过外源添加法研究酚酸物质对玉米大斑病菌丝生长和孢子萌发的影响。结果表明5种酚酸物质抑制玉米大斑病菌丝生长,抑菌率在14.5%~100%之间,且随浓度的增加作用趋势增强;苯甲酸和邻苯二甲酸在0.125~1 mg/mL浓度下抑制孢子萌发;对羟基苯甲酸在0.0625~1.0 mg/mL浓度下促进孢子萌发。通过田间调查发现,在开始发病时,秸秆还田会抑制玉米大斑病病情指数的发展。
英文摘要:
      In recent years, corn straw returned has become common in farming of agricultural production. In this study,crop straw was decomposed and filtered at different time. Effect of crop straw decomposed liquid on mycelium growth and spore germination of Exserohilum turcicum was tested.The phenolic compounds of crop straw decomposed liquid were identified by HPLC and five phenolic compounds including p-hydroxybenzoic acid, phthalic acid, vanillic acid, syringic acid and benzoic acid were observed. Effects of the five phenolic acids on mycelium growth and spore germination of Exserohilum turcicum were tested. The five phenolic compounds which inhibited the mycelium growth to some degree produced 14.5 to 100 percent inhibition and the process was stronger with the in crease of concentration. Benzoic acid and phthalic acid inhibited the spore germination at concentrations of 0.125 to 1.0 mg/mL. P-hydroxybenzoic acid promoted the spore germination at concentrations of 0.0625 to 1.0 mg/mL. At the beginning of disease, straw returned inhibited the disease index of northern leaf blight of corn.
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