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[摘要]
汇总国内外单倍体育种研究理论与经验,经美国著名单倍体育种家张铭堂指导,组装集成出本规范,供试用参考。本规范制定了杂交诱导单倍生殖方法选育自交系的基本操作程序,将单倍体杂交诱导、子粒鉴选、植株确认、自然与人工加倍及双单倍体获得5个环节作出技术规定。在每个环节都还有技术关键(特别是人工加倍等多属商业机密范畴)在试用与待熟化。经多年实践验证,按此规范程序,只需2~3个生育世代即可批量选育出遗传高度纯合自交系。
[Key word]
[Abstract]
By summaried the theoretical and empirical of haploid breeding at home and abroad, and following the guidance of the well-known haploid breeder in the USA, this specification were assemblied for the trial information. The specification laid down the basic operating procedures of breeding inbred lines with hybridization-induced haploid, made the technical requirements in haploid hybridization-induced, seed screening, plant recognition, natural and artificial double and doubled haploid. The key technology in every aspect were trialing and not yet perfect(artificial doubling and many other areas was commercial confidentiality). Following this program, batch selection the high-volume homozygous inbred lines only needs 2 - 3 reproductive generations.
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