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利用核心SNP标记划分辽宁省常用玉米自交系 杂种优势群的研究
Heterotic Grouping of Maize Inbred Lines widely used in Liaoning Province by Core SNP Markers
投稿时间:2017-07-17  修订日期:2017-07-17
中文关键词:单核苷酸多态性  杂种优势群  遗传相似度  聚类分析
英文关键词:Single Nucleotide Polymorphism  Heterosis Croup  Genetic Similarity  Cluster Analysis
基金项目:辽宁省科技攻关项目(2015103001):基于DOUGLAS基因型分析平台的玉米SNP标记体系的构建与优化
作者单位E-mail
姜思奇 沈阳农业大学 2050919016@qq.com 
郭瑞 沈阳农业大学  
张敖 沈阳农业大学  
赵艳贺 河北省承德市滦平县北山新区农牧局  
时免免 沈阳农业大学  
邓丽霞 沈阳农业大学  
崔震海 沈阳农业大学  
阮燕晔 沈阳农业大学 yanyeruan@syau.edu.cn 
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中文摘要:
      利用三个密度的SNP标记对44份玉米自交系进行杂种优势群划分,其中包括8份经典对照自交系和36份辽宁省常用育种自交系。利用56k玉米芯片共筛选得到了46899个高质量的SNP标记,作为高密度SNP标记划分杂种优势群。然后,筛选了1008和101个SNP分别作为中密度和低密度SNP标记用于分群。结果表明,部分自交系的遗传相似度达到90%以上,不宜作为不同自交系进行育种应用。三个密度的SNP标记都有效地将待测玉米自交系划分为四个杂种优势群,分别为瑞德群、兰卡斯特群、旅大红骨与唐四平头混合群以及类PH4CV群。对比高密度SNP标记,利用中低密度SNP标记划分的杂种优势群内部分自交系间的遗传距离发生变化,不能精确解析杂种优势群群内自交系的亲缘关系。建议划分杂种优势群可以采用中低密度的SNP育种芯片,既节约成本又不失精度,但群内自交系亲缘关系的区分应该采用高密度SNP芯片,以保障其真实性。
英文摘要:
      In this study, 44 samples involving 8 contrast inbred lines of classic heterosis group and 36 common inbred lines in maize breeding of Liaoning province were grouped by the single nucleotide polymorphism (SNP) of three densities. A total of 46899 high-quality SNP markers were obtained from 56k Maize Genome Array platform as high density for heterosis grouping. 1008 and 101 core SNPs were selected as medium and low density for grouping, respectively. A part of lines should not use as the difference of breeding lines since genetic similarity was more than 90%.Cluster analysis with high-density SNPs classified the inbred lines into Reid, Lancaster, mix group of Lvda Rad Cob and Tang Siping head group, like PH4CV group. A similar result was obtained in cluster analysis with 1008 and 101 core markers, however, the genetic distances between a few of the inbred lines have changed. It is suggested that low or medium-density SNP cluster Heterosis grouping economically and effectively.When it demand to distinguish the relationship between the inbreed lines ,the high-density SNPs should be selected for realness.
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