• 首页期刊简介获奖情况征稿简则刊登广告欢迎订阅联系我们网通电信
玉米自交系X178、掖478授粉前后miRNA表达差异分析
Differential Analysis of miRNA Expression before and after Pollination in Maize inbred Lines X178 and Ye478
投稿时间:2017-08-28  修订日期:2018-01-11
中文关键词:玉米  microRNA  深度测序  RNA seq  差异分析
英文关键词:Maize  MicroRNA  Deep sequencing  RNA seq  Difference Analysis
基金项目:国家科技支撑计划(2014BAD01B01),“十三五”国家重点研发计划(2017YFD0300703)
作者单位邮编
王琳琳. 沈阳农业大学特种玉米研究所
中国农业科学院作物科学研究所 
110866
赵永平 中国农业科学院生物技术研究所 
张从省. 安徽农业大学生命科学院
中国农业科学院作物科学研究所 
杨海龙 中国农业科学院作物科学研究所 
王宏伟 沈阳农业大学特种玉米研究所 110866
谢传晓 中国农业科学院作物科学研究所 
摘要点击次数: 237
全文下载次数: 63
中文摘要:
      microRNA(miRNA)是一类新型小分子非编码单链RNA,长度约22个碱基,在植物生物学方面起着重要作用[1]。本实验基于HiSeq高通量测序技术并结合生物信息学方法对X178、掖478两种材料授粉前后两个时期穗已知miRNA及靶基因进行分析,发现X178授粉前后差异表达miRNA有100个,分别属于21个miRNA家族;掖478授粉前后共筛选出98个有差异的miRNA,分别属于22个miRNA家族。利用miRNA与靶基因mRNA负调控关系初步确定授粉前后miRNA可能调控的mRNA靶点,建立miRNA-靶mRNA一一对应关系。结果表明,X178中上调表达的13个miRNA对应11个下调表达靶基因,下调表达的33个miRNA对应41个上调表达靶基因;掖478中上调表达的5个miRNA对应7个下调表达靶基因,下调表达的28个miRNA对应33个上调表达靶基因。其中6个miRNA家族共靶向11种mRNA。以上研究结果表明,在授粉前后玉米穗中已知miRNA在不同玉米自交系中种类具有差异。
英文摘要:
      microRNA (miRNA) is a new class of approximately 22 nt small-molecule and non-coding single strand RNA, which plays key role in plant biology. Combined with bioinformatic method, the target genes of known miRNA in X178 and Ye478 were analyzed by HiSeq high-throughput sequencing technique with two periods of ear before and after pollination. The results indicated that 100 miRNAs of X278 before and after pollination were different expression , which were attributed to 21 miRNAs families and 98 miRNAs of Ye478 before and after pollination were different expression, which were attributed to 22 miRNAs families. By using the negative regulation relationship between miRNA and target mRNA to preliminary determination miRNA possible regulatory mRNA target before and after pollination, then we establish correspondence between miRNA-target mRNA. The results revealed that 13 miRNAs up-regulation expressed correspond to 11 down-regulation expression target genes and 33 miRNAs down-regulation expressed correspond to 41 up-regulation expression target genes in X178. 5 miRNAs up-regulation expressed correspond to 7 down-regulation expression target genes and 28 miRNAs down-regulation expressed correspond to 33 up-regulation expression target genes in Ye478. Including 6 miRNAs families target to 11 mRNA. The results of this study show that the known miRNA of ear before and after pollination have different kind in different maize inbred lines.
查看全文   查看/发表评论  下载PDF阅读器