| Genome-wide association were conducted using phenotypic data from three environments (Sanya, Hainan; Xinxiang, Henan; Shunyi, Beijing) on a panel of 365 inbred lines and a set of 55K single-nucleotide polymorphism (SNPs) generated by Chip. The results showed that plant height varies very abundantly in three environments, and tends to increase gradually from low latitude to high latitude. Plant height has extremely significant correlation in all three environments. Variance analysis showed that plant height has extremely significant differences in genotype, environment, genotype and environment interaction. Genome-wide association study showed that significant association sites with plant height were detected in different environments. Significant association SNPs were found on chromosomes 1, 3, 5, 6, 7 and 8. In Xinxiang and Shunyi environments, Significant association SNPs were found in the bin5.05 region. In Hainan and Shunyi environments, significant association SNPs were found in the bin6.05 region. By comparing the significant SNP loci in association analysis with the reference genome, 22 most likely candidate genes were obtained in the MaizeGDB database . Once these SNPs are validated, they will be useful for marker-assisted selection and for a better understanding of maize plant height.