| A recessive ligamentless mutant was found in the self-crossing population of a maize inbred line Huangzao4 in 2008, and then a homozygous and stable hereditary ligamentless inbred line (Huangzao4lg) was gained through continual self-crossing. In order to explore and make full use of the elite genetic resources of maize ligamentless trait, and to improve population production of maize, five ligamentless maize inbred lines (JiV203 lg, K10 lg, JiV088 lg, JiV152 lg and Zhe461 lg) were obtained by back-crossing the inbred lines(JiV203, K10, JiV088, JiV152 and Zhe461) as recurrent parents to Huangzao4lg as donor parent, respectively. That is, 5 near isogenic lines of maize ligamentless trait were obtained, too. In this study, 5 pairs of near-isogenic lines were used as the mapping population, which was genotyped by Affymetrix 5H90K SNP chip, and then the gene of the ligamentless trait was mapped. In addition, candidate genes were primarily analyzed according to B73 reference sequence. The results showed that the maize ligamentless gene was located in the physical region of about1.12 Mb on the 2.01-2.02 bins, and there are 64 genes totally in this interval, of which 28 genes encoded functional proteins and 38 genes encoded putative proteins and unknown proteins,.