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利用近等基因系对玉米无叶舌基因的定位
Gene Mapping of Maize ligamentless trait Using Near Isogenic Lines
投稿时间:2018-11-23  修订日期:2019-10-09
中文关键词:玉米  无叶舌  近等基因系  基因定位
英文关键词:maize  ligamentless  near isogenic lines  gene mapping
基金项目:吉林省农业科学院创新工程研究生基金项目
作者单位E-mail
张叶 吉林省农业科学院 zhangy4943@163.com 
张艳 吉林省农业科学院  
邢跃先 吉林省农业科学院  
王梓钰 吉林省农业科学院  
闻竞 吉林省农业科学院  
郝东云 吉林省农业科学院  
李世界 吉林省农业科学院  
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中文摘要:
      本课题组于2008年在黄早四的自交群体中发现了隐性遗传的无叶舌突变株,后经过连续自交后育成纯合一致、稳定遗传的无叶舌自交系(黄早四lg)。随后为了充分挖掘并利用玉米无叶舌优异的基因资源,致力于提高玉米的群体产量,分别以5个玉米骨干自交系(吉V203、K10、吉V088、吉V152和哲461)为轮回亲本,黄早四lg为供体亲本,进行回交转育,并获得相应的无叶舌自交系吉V203lg、K10lg、吉V088lg、吉V152lg和哲461lg,即同时获得5对玉米无叶舌性状的近等基因系(Near Iso-genic Lines, NIL)。本研究以5对近等基因系为定位群体,利用Affymetrix 5H90K基因芯片对5对玉米无叶舌近等基因系进行基因型检测,进而对玉米无叶舌基因进行定位,并在此基础上对候选基因进行初步分析。结果表明本研究中的玉米无叶舌基因位于2.01-2.02bin上,约1.12Mb的物理区间内(根据B73参考基因组),在此区间内共共有64个基因,其中26个基因编码功能蛋白,有38个未知功能的蛋白和假定蛋白。
英文摘要:
      A recessive ligamentless mutant was found in the self-crossing population of a maize inbred line Huangzao4 in 2008, and then a homozygous and stable hereditary ligamentless inbred line (Huangzao4lg) was gained through continual self-crossing. In order to explore and make full use of the elite genetic resources of maize ligamentless trait, and to improve population production of maize, five ligamentless maize inbred lines (JiV203 lg, K10 lg, JiV088 lg, JiV152 lg and Zhe461 lg) were obtained by back-crossing the inbred lines(JiV203, K10, JiV088, JiV152 and Zhe461) as recurrent parents to Huangzao4lg as donor parent, respectively. That is, 5 near isogenic lines of maize ligamentless trait were obtained, too. In this study, 5 pairs of near-isogenic lines were used as the mapping population, which was genotyped by Affymetrix 5H90K SNP chip, and then the gene of the ligamentless trait was mapped. In addition, candidate genes were primarily analyzed according to B73 reference sequence. The results showed that the maize ligamentless gene was located in the physical region of about1.12 Mb on the 2.01-2.02 bins, and there are 64 genes totally in this interval, of which 28 genes encoded functional proteins and 38 genes encoded putative proteins and unknown proteins,.
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