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基于SNP芯片揭示糯玉米种质资源遗传多样性
Genetic Diversity of Waxy Maize Germplasm Revealed by SNP-Chips
投稿时间:2019-01-21  修订日期:2019-07-16
中文关键词:糯玉米  SNP标记  遗传多样性
英文关键词:Waxy maize  SNP marker  genetic diversity
基金项目:上海市种业发展项目[沪农科种字(2016)第1-3号], 上海市科学技术委员会科研计划项目(153919N1300, 17391900100, 17391902702), 上海市农业科学院卓越团队项目(农科创2017B-08), 上海市市级农口系统青年人才成长计划[沪农青字(2015)第1-4号], 上海市市级农口系统青年人才成长计划[沪农青字(2018)第1-6号], 2019上海市农业科学院青年科技人员“助跑”计划。
作者单位E-mail
卢媛 上海市农业科学院 jenny_paper@163.com 
韩晴 上海市农业科学院  
艾为大 上海市农业科学院  
施标 上海市农业科学院  
王义发 上海市农业科学院  
潘春丹 上海市浦东新区农业技术推广中心  
沈雪芳 上海市农业科学院  
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中文摘要:
      为了提高糯玉米种质资源的利用效率,挖掘优异糯玉米种质资源、改良糯玉米品种。利用SNP(Single nucleotide polymorphism)芯片技术对44份不同来源的糯玉米自交系和5份分别代表中国玉米5大类群的普通玉米自交系进行全基因组扫描,34 257个SNP 标记在49份玉米自交系中的多态性信息含量(polymorphism information content, PIC) 为0.02~0.56,平均含量为 0.32;最小等位基因频率(minor allele frequency, MAF) ≥ 0.03。49 份玉米自交系间的状态一致性(identity-by-state, IBS)变幅为0.59~0.99,平均为0.68。基于IBS利用 Neighbor-joining (NJ) 聚类分析方法,将 49 份自交系划分为5个类群,分别包含 19 份、9 份、4份、9 份和 3 份材料,其中系谱来源不清晰的糯玉米自交系被划分至不同类群,明确了它们的亲缘关系。本研究为糯玉米杂种优势利用、种质资源创新和遗传进化分析提供了材料基础和理论依据。
英文摘要:
      In order to improve the utilization efficiency of waxy maize germplasm in excellent germplasm resources exploitation and waxy maize variety improvement. A genome-wide scan was performed on 5 crop maize inbred lines, which represent crop maize germplasm resources in China respectively, and 44 waxy maize inbred lines from different sources with SNP-Chips. A total of 34,257 SNPs with Polymorphic Information Content (PIC) ranged from 0.02 to 0.56, with an average of 0.32; and a minimum allele frequency (MAF) ≥ 0.03 were used for genetic diversity analysis. The identity-by-state (IBS) of the 49 inbred lines ranged from 0.59 to 0.99 with an average value of 0.68. 5 groups were divided from 49 maize inbred lines by Neighbor-joining (NJ) clustering method based on IBS, including 19, 9, 4, 9, and 3 materials. Waxy maize inbred lines with unknown pedigree were divided into different groups, so their relatedness with other inbred lines was defined. This study provides basic materials and a theoretical basis for heterosis utilization, germplasm innovation and evolutionary genetics analysis of waxy maize.
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