[关键词]
[摘要]
利用Illumina HiSeq技术,分别构建5℃和22℃处理下的两个玉米叶组织转录组。从转录组数据中共分离出38条低温响应NAC基因,包括6条膜结合转录因子;38条NAC中30条为上调,8条为下调。38条ZmNACs被分为12个亚族(NAC a-1),其中,7条上调低温响应ZmNACs属于逆境相关亚家族(Stress related/NAC-h)。同一亚家族内,蛋白结构域和内含子/外显子结构相对保守。不同ZmNAC基因的在不同组织部位和发育阶段的表达模式有很大变化。许多重要的非生物胁迫相关元件在38条ZmNACs的启动子中高度富集。
[Key word]
[Abstract]
NAM, ATAF1, -2, and CUC2(NAC) proteins are plant-specific transcription factors that play impor-tant roles in different developmental processes and stress responses. In this study, Illumina HiSeq technology was used to construct two transcriptomes of maize leaf tissue treated at 5and 22℃, respectively. From the transcriptome data, 38 low-temperature response NAC genes were isolated, including 6 membrane-bound transcription factors. Among the 38 NACs, 30 were up-regulated and 8 were down-regulated. Thirty-eight ZmNAC were divided into 12 subfamilies (NAC a-1), of which 7 up-regulated low-temperature response ZmNAC belonged to stress related/NAC-h. Within the same subfamily, protein domains and intron/exon structures are relatively conserved. The ex-pression patterns of different ZmNAC genes at different tissue sites and at developmental stages vary greatly. Many important elements in abiotic stress are highly enriched in the promoters of 38 ZmNAC. This study will help deep-en the understanding of the maize NAC gene family and provide clues for further functional studies.
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[基金项目]
国家自然科学基金项目(31701328)、黑龙江省自然科学基金项目(QC2016036)、黑龙江省博士后资助经费(LBHZ16165)、黑龙江八一农垦大学青年创新人才项目(CXRC2016-03)、黑龙江八一农垦大学学成引进人才科研启动计划项目(XYB2015-02)、黑龙江省教育厅科学技术研究项目(12541575)、黑龙江八一农垦大学研究生创新科研项目(YJSCX-2017-Y01)