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低磷胁迫下玉米幼苗生理响应及相关基因表达研究
Physiological Response and Related Genes Expression of Maize Seedlings under Low Phosphorus Stress
投稿时间:2019-03-14  修订日期:2019-08-13
中文关键词:玉米  磷胁迫  光合作用  转录组  
英文关键词:Maize(Zea mays)  Phosphorus stress  Photosynthesis  Transcriptome  
基金项目:科技部国家重点研发计划“七大农作物育种” 项目(2016YFD0101002);黑龙江八一农垦大学引进人才科研启动基金((2031011047);黑龙江八一农垦大学研究生创新项目(YJSCX2017-Y03);
作者单位E-mail
王枫 黑龙江八一农垦大学农学院 huhuwf0218@163.com 
王玉凤 黑龙江八一农垦大学农学院  
许晓萱 黑龙江八一农垦大学农学院  
李嘉欣 黑龙江八一农垦大学农学院  
丁冬 黑龙江八一农垦大学农学院  
贺琳 黑龙江八一农垦大学农学院  
李佐同 黑龙江八一农垦大学农学院  
徐晶宇 黑龙江八一农垦大学农学院  
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中文摘要:
      以玉米品种合344为试验材料,对低磷胁迫下玉米幼苗叶片生理表型指标与玉米叶片转录组差异基因表达数据联合分析,探究低磷胁迫下与玉米幼苗叶片生理表型变化相关的关键响应基因及其调控规律。结果表明:低磷胁迫下玉米叶片的叶绿素荧光参数(FV/FM)以及叶绿素含量均有不同程度的降低,并且叶绿素合成相关基因大部分为下调表达,叶绿素降解相关基因大部分为上调表达。玉米叶片光合特性相关基因大部分呈下调表达,同时光合效率显著降低。在低磷胁迫下,SOD与POD活性随处理时间增长逐渐上升,编码两种酶的基因大部分上调表达。同时检测到8条MDA合成关键基因LOX,磷胁迫下其中7条均有不同程度的上调表达,同时MDA含量也随处理时间逐渐上升。
英文摘要:
      The maize variety 344 was used as the experimental material, and the physiological characteristics data and the transcriptome differential gene expression data of maize seedlings under low phosphorus stress were analyzed. To deciphers the molecular regulation of physiological changes in maize Leaves under low phosphorus stress, and preliminarily identified the key genes involved in physiological response under low phosphorus stress. The results showed that under low phosphorus stress, chlorophyll fluorescence parameters (FV/FM) and chlorophyll content of maize leaves decreased in varying degrees, chlorophyll synthesis related genes were mostly down-regulated, and chlorophyll degradation related genes were mostly up-regulated. Most of the genes related to photosynthetic characteristics of maize leaves were down-regulated, and photosynthetic efficiency was significantly reduced. Under phosphorus stress, the activities of SOD and POD increased gradually with the increase of treatment time, and most of the genes encoding the two enzymes were up-regulated. At the same time, eight key genes of MDA synthesis LOX were detected,and seven of them were up-regulated under phosphorus stress. Meanwhile, the content of MDA increased gradually during low phosphorus treatment.
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