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玉米热激蛋白ZmHSP70-12基因的克隆及表达分析
Cloning and Expression Analysis of Heat Shock Protein Gene ZmHSP70-12 in Maize
投稿时间:2019-05-09  修订日期:2019-08-25
中文关键词:玉米  热激蛋白  ZmHSP70-12  亚细胞定位  非生物胁迫
英文关键词:maize(Zea mays L.)  heat shock protein  ZmHSP70-12  subcellular Localization  abiotic stress
基金项目:
作者单位E-mail
王帅 河北农业大学 河北省植物生理与分子病理学重点实验室 1830063118@qq.com 
祁茂冬 河北农业大学 河北省植物生理与分子病理学重点实验室  
魏凤菊 河北农业大学 河北省植物生理与分子病理学重点实验室  
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中文摘要:
      热激蛋白70(heat shock protein 70,HSP70)是原核和真核细胞中普遍存在的一种高度保守的分子伴侣。本研究从玉米中克隆了一个HSP70家族成员。该基因cDNA序列全长为1992 bp,开放阅读框为2352 bp,编码663个氨基酸,蛋白质分子量约75.0 kD,蛋白结构预测及同源比对分析表明,该基因编码蛋白含ATPase位点和HSP70保守结构域,与拟南芥AtHSP70-12序列高度相似,遂命名为ZmHSP70-12。蛋白亚细胞定位显示,ZmHSP70-12蛋白在内质网中表达。实时荧光定量PCR分析表明,ZmHSP70-12对非生物胁迫高温、干旱均具有明显的应答反应,从而推测ZmHSP70-12是玉米中与胁迫逆境相关的基因。
英文摘要:
      Heat shock protein 70 (HSP70s) are found extensively in prokaryotic and eukaryotic and one of the most conserved molecular chaperone. In this study, a member of the HSP70 family was isolated from maize. It’s full-length coding sequence was 1992bp, and it’s open reading frame is 2352bp. The encoded protein with molecular weight is about 75.0kD.Through protein structure prediction and homologous alignment analysis, the gene encodes a protein containing ATPase site and HSP70 conserved domain. It is highly similar to Arabidopsis AtHSP70-12 sequence, so it is named ZmHSP70-12. Subcellular localization analysis showed that the ZmHSP70-12 protein was expressed in the endoplasmic reticulum. Real-time quantitative PCR analysis revealed ZmHSP70-12 has obvious response to abiotic stress, such as high temperature and drought.Therefore, it was speculated that ZmHSP70-12 is a stress-related gene in maize stress.
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