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玉米穗轴对穗粒腐病抗性鉴定体系与优异抗源的研究
Study on Cob Inoculation System and Elite Resistant Inbred Lines Screened to Maize Ear Rot
投稿时间:2019-07-04  修订日期:2019-09-18
中文关键词:玉米穗粒腐病  穗轴抗性  鉴定指标  优异抗源
英文关键词:Maize Ear Rot  Resistance of Cob  Identification index  Elite Inbred Lines
基金项目:国家自然科学(31671281)、国家自然科学(31761143009)
作者单位E-mail
王昭 河南农业大学农学院 475356602@qq.com 
穆聪 河南农业大学农学院  
李云梦 河南农业大学农学院  
高景阳 河南农业大学农学院  
宋云霞 河南农业大学农学院  
董朝沛 河南农业大学农学院  
马培培 河南农业大学农学院  
赫可伟 河南农业大学农学院  
许静 河南农业大学农学院  
董华芳 河南农业大学农学院  
孙小东 河南农业大学农学院  
韩亚楠 河南农业大学农学院  
周子健 河南农业大学农学院  
陈甲法 河南农业大学生命科学学院  
吴建宇 河南农业大学农学院  
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中文摘要:
      穗轴作为拟轮枝镰孢菌侵染果穗引发玉米穗腐病的重要途径之一,能够影响病原菌的侵染和扩散。为了深入开展玉米穗轴对拟轮枝镰孢菌抗性的研究以及了解玉米穗腐病籽粒与穗轴抗性的差异,本研究进行了抗性鉴定体系的优化,并进一步利用164份国内外玉米骨干自交系进行了两年两点的接种鉴定筛选优异抗源。通过对发病边长、发病芯长、发病面积、发病相对面积等4个抗性指标进行研究,发现其中发病边长能较大程度地区分不同自交系间的抗感差异且又在材料内具有较好的重演性,因此把发病边长确定为玉米穗腐病穗轴抗性鉴定指标,进而对参试自交系进行了抗性评价,鉴定出CML27、BT-1、CML274等58份穗轴高抗自交系,同时筛选出BT-1、CML173、CML193等8份籽粒与穗轴均抗穗腐病的优异抗源,为玉米穗腐病抗性遗传研究以及抗病育种工作奠定了基础。
英文摘要:
      As an important pathway, cob can affect the infection and spread of Fusarium verticillioide in maize ear. In order to study the cob resistance to Fusarium verticillioide and to understand the difference between kernel resistance and cob resistance in maize, the identification system of cob resistance was optimized. In addition, 164 inbred lines were surveyed to establish the cob resistance identification system and to select excellent resistance inbred lines in two years and two locations. By analyzed of the four indexes (surrounding infected length, internal infected length, infected area and relative infected area), the surrounding infected length was identified as optimal, which has better repeatability in same inbred line and can easier distinguish the resistance between different inbred lines. Fifty-eight inbred lines with high resistance of cob were identified, such as CML27, BT-1, CML274, etc. At the same time, elite inbred lines BT-1, CML173, CML193 and other eight inbred lines with high resistance to ear rot in both kernel and cob were screened out. These results laid a foundation for disease resistance breeding and resistance inheritance research.
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