[关键词]
[摘要]
玉米杂交诱导单倍体机制还不十分明确,多数学者支持无融合生殖——假受精的单倍体诱导机制假说。通过研究异雄核受精对杂交诱导单倍体的影响,发现在单倍体诱导过程中发生了受精的延迟,而受精的延迟是引起较高异雄核受精率的原因,进而导致诱导率下降。不同诱导系诱导同一自交系产生的单倍体之间存在显著差异,推测这种差异可能源自于与诱导系的部分杂交。通过这些与上述诱导机制不一致的结果,对杂交诱导单倍体机理进行了进一步探析。
[Key word]
[Abstract]
Recently, haploidy has been used widely in both maize breeding and genetics. However, the mechanism of haploid induction has not been explained, so far. In this work, we would like to present some results which have some contradictions with the mechanism of haploid induction which is known in apomictic development-pseudogamy. We also study the influence of heterofertilization on the induction of maternal haploids. The results indicated that during the induction of haploids adelay of fertilization occurs. In our opinion, the delay of fertilization was the reason for the high frequencies of heterofertilization in the pollinations where the haploid inducer was used. A delay of pollination leads to a decrease in the frequency which could also be caused by the phenomenon of heterofertilization. The last goal was to compare adult haploids of inbred lines produced by different inducers. It seems most likely the reason for the differences between haploids was partial hybridization with the inducers.
[中图分类号]
[基金项目]
高产转基因玉米新品种培育(2008ZX08003-003)、“973”计划“高产优质育种新材料的创制”(2009CB118400)、“863”计划“强优势玉米杂交种的创制与应用”