[关键词]
[摘要]
以玉米自交系L26和095组配的F2世代为作图群体,采用SSR分子标记技术和复合区间作图法对玉米茎粗等7个株型性状进行基因定位。共检出21个QTL,其中茎粗检测到1个位点(qSD1),穗位高、株高均检测到3个QTL位点(qEH1-qEH3、qPH1-qPH3),雄穗分枝数检测到5个QTL位点(qTBN1-qTBN5),叶片数检测到4个QTL位点(qLN1-qLN4),叶型系数检测到3个QTL位点(qLSC1-qLSC3),叶向值检测到2个QTL位点(qLOV1-qLOV2)。21个QTL中,qTBN1、qTBN4、qLN1、qLN3、qLN4这5个QTL解释表型变异率超过30%,表现出明显的主效QTL效应。研究还发现,有5个影响不同性状的QTL位于染色体上相同标记区间内或与相同标记连锁,分为Ch3-2和Ch8-1两个区段,表现出了成簇分布的特性。
[Key word]
[Abstract]
In this paper, 21 QTLs, related to plant height, ear height, stem diameter, tassel branch number, leaf shape coefficient, leaf number, and leaf orientation value were located on the maize linkage map constructed by F2 population of L26 × 095 through SSR genotyping. By using the CIM(composite interval mapping) algorithm, 1 - 5 QTLs were identified for each of the above seven plant-type-related traits. Among the 21 QTLs, qTBN1, qTBN4, qLN1, qLN3 and qLN4 accounted for over 30 percent of the phenotypic variance, indicating a significant characteristics of major genes, and these 21 QTLs distributed uniformly among the 10 maize genomes. Additionally, three QTLs related to ear height(qEH2), plant height(qPH2), and leaf number(qLN2) were all located between bnlg1257 and umc2277, and two QTLs related to tassel branch number(qTBN5) and leaf orientation value(qLOV2) were both located nearby the marker of mmc0001, which indicated that some QTLs controlled related phenotypic traits tended to be clustered together.
[中图分类号]
[基金项目]
重庆市“水稻玉米良种创新”重大专项(CSCT,2007AA 1017)