以不同品种甜玉米和糯玉米幼胚为供试材料，研究了不同培养基对愈伤组织诱导、继代和分化的影响，并在高温条件下筛选玉米耐热性变异体。结果表明：采用含有低浓度2,4-D的N₆为玉米幼胚诱导培养基，可产生一定比例的变异，愈伤组织诱导率高，质量较好；MS培养基适于分化；温度渐变法可以保证幼胚能够在产生耐热变异的同时不至于死亡，并在最后进行了恢复培养,即温度恢复到适温，淘汰不稳定愈伤组织，得到相对稳定的耐热性变异体；通过测定愈伤组织游离脯氨酸浓度、再生植株叶片质膜相对透性和叶片相对失水率，确定玉米胚变异体的耐热程度。

The paper induced callus from immature embryos of sweet corn and waxy corn. The effect of media on the callus induction, subculture, quality of embryogenic callus and the frequency of plantlet regeneration were studied. The paper also cultured callus under different high temperature to improve heat tolerance of maize callus. The results showed that the high quality and high induction rate of celli were obtained when N₆ was used as the induction medium. At the same time, the frequency of callus induction could be greatly improved when 2 mg/L 2,4-D were added, and it also generates a few variations. MS was suitable for regeneration of callus. In the period of callus induction, temperature should be raised step by step to a high of 40℃ and remain for 12 h. Finally return to the original environmental temperature. This way can help callus improve heat tolerance, with keeping health. The paper analyzed the degree of heat tolerance of these treated calluses by testing concentration of proline and ion conduction rate.

广东省农业厅“优质蛋白玉米品种和耐旱玉米品种的引进”[粤财农(2005)117]、“耐寒性糯玉米良种的引进筛选与技术配套”项目(2007A020300002-3)