[关键词]
[摘要]
以引进的35个糯玉米自交系和5个我国普通玉米杂种优势群的标准检验种为供试材料,利用SSR标记分析他们的遗传多样性和亲缘关系.结果表明,30对SSR引物共检测到140个等位基因变异,每对引物的等位变异数为2~8个,平均为4.67个;SSR标记的多态性信息量在0.198 6~0.794 7之间,平均为0.584 0;材料间的遗传距离在0~0.923 1之间,平均值为0.645 3.40份材料可以分为4个类群,与可追踪的系谱信息基本一致.
[Key word]
[Abstract]
Thirty-five waxy maize germplasms and five standard test inbred lines represented for 5 maize heterotic group in China were analyzed by using SSR markers. The results showed that a total of 140 alleles were detected by using 30 pairs of SSR primers, the number of alleles per SSR locus ranged from 2 to 8, with and average of 4.67; the polymorphism information content of SSR markers ranged from 0.198 6 to 0.794 7, with an average of 0.584 0; the pair-wise genetic distance between 40 accessions ranged from 0 to 0.923 1, with an average of 0.645 3. Cluster analysis showed that 40 accessions of waxy and common maize germplasms could be divided into 4 groups, consistent with the pedigree information traceable.
[中图分类号]
[基金项目]
现代农作物种业发展专项资金项目"吉林省主要农作物种质资源、育种方法及制种技术研究"、吉林省财政厅科研育种项目"糯玉米新种质创制"