The physiological races of 356 strains of Bipolaris maydis were identified, which were collected from Hebei province from 2007 to 2016. Results showed that isolation frequencie of Race T, Race C, Type S and Race O is different from year to year. Race O is the dominant race of of B.maydis in Hebei province with 94.94% everage isolation frequence. Meanwhile, the pathogenicity of race O to hybrid Zhengdan 958 and inbred line C103 was declining. However, the pathogenicity of Bipolaris maydis to C103 decreased less than that to zhengdan 958. ITS sequence of 43 strains collected from Shijiazhuang, Baoding, Hengshui, Cangzhou Hebei Province, Shenyang Liaoning Province and Jinan Shangdong Province were analyzed and UPGMA evolutionary Tree was construction .The result indicated that the genetic evolution of Bipolaris maydis was weakly geographically related.