Over the past decade, agrobacterium mediated genetic transformation has been widely used in breeding and molecular biology research because of its stability and efficiency. However, when using traditional agrobacterium mediated transformation in corn, the transformation of agrobacterium tumefaciens is limited, and it has many disadvantages, such as complex operation, low conversion rate, and so on. Biologists have found that the use of ultrasonic-assisted agrobacterium-mediated transformation in germinating corn seed can improve the agrobacterium mediated transformation rate. In this study, the ultrasonication-assisted Agrobacterium treatment method was modified with the addition of emery to increase the treatment intensity to the apical meristem cells of germinating seeds. The emerged 93 plants from 800 control seeds were sprayed three times with glyphosate solution, and 27 of them survived, their leaves were taken with a PCR electrophoresis test and 23 shown positive, which suggested that epsps gene was introduced into the maize cultivar, and the transformation rate was 29%. Finally, which has been shown that the method has the advantages of circumventing the tedious tissue culture procedures and being easy to apply.