In this study, qRT-PCR and ELISA methods were used to detect the Cry 1Ab-Ma expression, and lab and field bioassay were applied to evaluate the insect resistance of transgenic Cry1Ab-Ma maize CM8101. The Cry1Ab-Ma genes could be detected to express in all tested tissues in CM8101, and there were no significant difference among different generations. Compared with non-transgenic maize Zheng58, CM8101 showed significant insect resistance to Ostrinia furnacalis in different tissues both in laboratory and field conditions. And no significant differences for the survival rate of the Ostrinia furnacalis were observed in these tissues among different generations. The resistance rate of CM8101 was detected to be Class 1 at each generation, showing high resistance level to Ostrinia furnacalis.