Used Gaoyou5 as haploid inducer, 8 elite double haploid inbred (DH) lines was selected from the foundation material of the hybrid generation F1 of M54×USA maize hybrid through haploid induction, natural doubling in field, cultivation identification and character selection. The combining ability and the application potential of the DH lines were analyzed by NCⅡ design. The results showed that the GCA was different from DH lines with the same genetic foundation. The DH lines of MDH4 and MDH8 had higher yield per plant GCA value, and it was easier to breeding high-yield hybrid with MDH4 or MDH8 as a parent. Comprehensive analysis of the performance of the main agronomic traits showed that the DH line of MDH4 was an ideal parent to breed high yield and high quality excellent maize hybrids, and the DH lines of MDH8 and MDH5 also had great application potential. MDH4×H451, MDH4×S121 and MDH8×H451 had high TCA and high yield per plant, which could be confirmed as good combinations and should be tested in multiple locations for many years in order to exploit their application potential. According to the estimate of genetic parameters, plant height, ear height, ear length and ear rows should be selected in early generation. When breeding maize hybrids, plant height, ear height, ear length and ear diameter should be focus on using the DH or inbred lines with outstanding performance of GCA as parents, ear rows, 1000-kernel weight and yield per plant should be given consideration to GCA of parents and SCA of hybrids, and the selection of SCA should be emphasized in improving kernel number. This research provides a reference for screening, identifying and utilizing DH lines and hybrids with application potential.