As an important pathway, cob can affect the infection and spread of Fusarium verticillioide in maize ear. In order to study the cob resistance to Fusarium verticillioide and to understand the difference between kernel resistance and cob resistance in maize, the identification system of cob resistance was optimized. In addition, 164 inbred lines were surveyed to establish the cob resistance identification system and to select excellent resistance inbred lines in two years and two locations. By analyzed of the four indexes (surrounding infected length, internal infected length, infected area and relative infected area), the surrounding infected length was identified as optimal, which has better repeatability in same inbred line and can easier distinguish the resistance between different inbred lines. Fifty-eight inbred lines with high resistance of cob were identified, such as CML27, BT-1, CML274, etc. At the same time, elite inbred lines BT-1, CML173, CML193 and other eight inbred lines with high resistance to ear rot in both kernel and cob were screened out. These results laid a foundation for disease resistance breeding and resistance inheritance research.