中性洗涤纤维(NDF)是衡量青贮玉米纤维质量重要指标之一。本试验以341 份玉米自交系为材料于2018年沈阳和通辽种植并收获,对其秸秆NDF含量测定。利用全基因组重测序获得高质量的6,276,612个SNPs用于全基因组关联分析,在所有染色体上共检测到49个显著SNPs位点(P＜0.000001),在显著水平P＜0.00000001上检测到10 个SNPs位点,在此基础上共找到8个和秸秆NDF关联的候选基因,其编码产物生物学功能主要涉及到细胞生长发育、胁迫应答、DNA修复损伤等。本研究对于秸秆NDF的遗传和分子机理研究以及青贮玉米的选育具有重要理论基础。
SNeutral Detergent Fiber (NDF) is an important index to measure the fiber quality of silage maize. In this study, 341 maize inbred lines were evaluated for NDF in 2018 in Shenyang and Tongliao experimental stations. These inbred lines were subjected to whole genome sequencing, generating a total of 6,276,612 high quality SNPs. GWAS analysis detected 49 SNPs using the threshold of P < 0.000001 and 10 SNPs using the threshold of P < 0.00000001. Eight candidate genes were identified based on the GWAS, the biological functions of these genes mainly involved in cell growth and development, stress response, DNA repair damage. These results would be useful for researching on the genetic and molecular mechanism of NDF, and for the development of silage maize cultivars.