Using 336 DH lines bred by haploid breeding from F1of PH4CV × A6 were male parents, and 336 hybrid combinations were made with ph6wc as female parent. Three repeated identification tests were carried out for 14 combinations with better comprehensive characters and Xianyu 335 (PH6WC × PH4CV) and Tongyu 99 (PH6WC × A6) after 2-year identification and elimination. Variance, genetic coefficient and cluster analysis were carried out for the additive dominant effect of 10 characters. The results showed that plant height, ear height, grain moisture, 100 grain weight and bulk density had super high parent and super low parent heredity, the ratio of grain to ear had super high parent heredity, and the grain weight per ear had no super high parent heredity. The effect of path coefficient of each character on the grain weight per ear was: 100 grain weight > ear row number > ear length > bulk density > plant height > ear height and the negative effect was (absolute value): grain moisture > grain ear ratio> bald tip length. 14 DH lines can be divided into 3 groups, The chi square distance of PH4CV and A6 is 3.45, and that of D752 and A6 is 1.96. In this round of haploid breeding practice, only D752 ear grain weight additive dominant effect should reach PH4CV level, while taking into account the low plant height, low ear height and other excellent characters, the improvement efficiency is 0.30%.