The induction rates of 80 new haploid induction lines were studied with three hybrids Jingke968, Xianyu335, Jingke528 and four inbred lines Jing724, Jing92, Jing X005 and Jing17 as test species. The results showed that there were errors in the identification of haploid kernel by means of grain color markers, which affected the identification accuracy, and the actual induction rate was obtained by the further correction in field planting. The haploid induction rates of different plants in the same inducer line and among different test species were different. Two hundred eighty-two test crosses combinations of 140 individual plants from 78 induced lines were analyzed. Among them, the actual induction rate of 25 test cross combinations of 21 induced lines was more than 15.0%, and the highest induction rate was 25.3%. Twelve haploid induction lines were screened for further study.