以拟南芥作为种子序列进行Blastp序列比对，共鉴定15个ZmGRFs基因，对其理化性质、系统进化关系等在非生物胁迫下的表达进行分析。根据系统发育树分析表明，ZmGRFs基因与单子叶植物水稻亲缘关系较近，15个ZmGRFs基因分别定位在1、2、4、5、6、7、9、10号染色体上且各个基因之间高度同源，每个亚族之间基因结构及保守基序均相对保守。ZmGRFs蛋白二级结构均以α-螺旋和无规卷曲为主。根据磷酸化位点预测分析表明，除ZmGRF6和ZmGRF9不含酪氨酸外，其他ZmGRFs蛋白均含有潜在的丝氨酸、苏氨酸和酪氨酸位点且各蛋白之间的潜在的磷酸化位点数目差异较大。根据转录组数据分析表明，ZmGRF4和ZmGRF13基因在NaHCO₃上调表达，同时发现热、盐和干旱胁迫下ZmGRF4和ZmGRF13基因表达较高，冷胁迫下ZmGRF1/3/4/15基因表达较高。根据不同组织部位表达谱分析显示，ZmGRF4和ZmGRF13在各个部位表达均较高。

Arabidopsis was used as a seed sequence for Blastp sequence alignment, a total of 15 ZmGRF genes were identified; and their physical and chemical properties, phylogenetic relationships, etc. were analyzed under abiotic stress expression. The results showed that according to phylogenetic tree analysis the relationship between GRF gene of maize and rice of monocotyledon plant was close; 15 ZmGRF genes were located on chromosomes 1, 2, 4, 5, 6, 7, 9 and 10 respectively, which there were highly homologous; gene structure and conserved motif function of each subgroup were relatively conservative; secondary structure of ZmGRF protein was mainly alpha-helix and random curl in maize; according to the prediction analysis of phosphorylation sites, except ZmGRF6 and ZmGRF9 not contain tyrosine, other ZmGRF proteins contain potential serine, threonine and tyrosine sites, and the number of potential phosphorylation sites varied greatly among proteins; transcriptome data analyzed the up-regulated expression of ZmGRF4 and ZmGRF13 genes under NaHCO₃ stress, meantime, ZmGRF4 and ZmGRF13 genes were found to have high expression levels under heat, salt and drought stress; ZmGRF1/3/4/15 gene expression was higher under cold stress; the expression profiles of different tissues were analyzed showed that ZmGRF4 and ZmGRF13 were highly expressed in various parts of maize.

黑龙江八一农垦大学优势特色学科建设项目专项（2042070002）