以玉米自交系Mo17为材料，用不同浓度的EBR处理受150mmol/LNaCl胁迫的玉米幼苗，测定其叶绿素含量、过氧化物酶（POD）活性、脯氨酸含量、可溶性糖含量、丙二醛（MDA）含量和根系活力等指标，同时通过荧光定量PCR测定CAT酶基因和ZMPIP2-4基因的表达。研究结果表明：（1）EBR处理后，玉米幼苗的POD酶活性、可溶性糖含量、脯氨酸含量和根系活力增加；MDA含量降低。（2）EBR处理浓度为0.1mg/L时，可溶性糖、过氧化物酶和根系活力显著增加。EBR浓度为0.001mg/L时，酶（CAT）基因的表达量有明显上升，但0.01mg/L及1.0mg/L时效果不显著，0.001mg/L和0.1mg/L处理时基因表达量有上升显著，0.001mg/L处理时效果最显著。 综上所述，EBR浓度为0.1mg/L时，在生理层面可有效增加玉米抗盐胁迫的能力；0.001mg/L时在基因层面可显著增加玉米幼苗抗盐胁迫CAT和ZMPIP2-4基因表达量。由此得出，有效缓解盐胁迫的最适EBR浓度为0.1mg/L。生理特性是基因的表型，然而玉米幼苗抗胁迫基因表达后调控具体机理尚不清楚，需进一步探究。

Maize inbred line Mo17 was used as material,and its seedlings under 150mmol/L NaCl stress were treated with different concentrations of EBR. Their chlorophyll content.Peroxidase (POD) activity, proline content, soluble sugar content, and malondialdehyde (MDA) content and root vigor were measured,while the expression of CAT enzyme gene and ZMPIP2-4 gene was measured by fluorescence quantitative PCR. The results of the study showed that: (1) After EBR treatment, the POD enzyme activity, soluble sugar content, proline content and root activity of corn seedlings increased; MDA content decreased. (2) When the concentration of EBR treatment was 0.1 mg/L, soluble sugar, peroxidase and root activity increased significantly. The expression of catalase (CAT) gene increased significantly at 0.001 mg/L , but the effect is not significant when treated with 0.01mg/L and 1.0mg/L. ZMPIP2-4 gene expression increased significantly after EBR treatment, with 0.001 mg/L treatment having the best effect, followed by 0.1 mg/L. In summary, when the EBR concentration is 0.1mg/L, it can effectively increase the salt stress resistance of corn at the physiological level; when the EBR concentration is 0.001mg/L, it can significantly increase the expression of CAT and ZMPIP2-4 genes in the salt stress resistance of corn seedlings at the genetic level. It can be concluded that the optimal EBR concentration to effectively alleviate salt stress is 0.1 mg/L. Physiological characteristics are the phenotypes of genes, but the specific mechanism of regulation after the expression of stress-resistant genes in maize seedlings is still unclear, so the further investigation is needed.

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