[关键词]
[摘要]
本文基于阻抗流式细胞技术,结合雄穗水培法及PCR仪模拟高温胁迫,建立花粉活力的快速测定体系,测定玉米自交系群体(227份)在常温(25℃)及模拟高温胁迫下(40℃/30min、50min、70min)的花粉活力值,利用BLUE模型估算综合花粉活力值,用于筛选耐高温型玉米自交系;并与果穗产量进行相关性分析。在常温下,花粉活力值在玉米自交系群体中变异丰富;随着模拟高温胁迫时间的延长,自交系间的花粉活力值存在不同程度的下降,其与小区果穗产量的相关系数逐渐减小,这表明玉米自交的花粉活力对高温胁迫的耐受性在群体中存在差异。利用BLUE模型估算模拟高温胁迫的综合花粉活力值,根据该值共筛选到耐高温型玉米自交系15份,它们的花粉活力均对高温胁迫具有较高的耐受性。本文为实现快速、定量评估玉米的花粉活力、评估玉米对高温胁迫的耐受性奠定了研究基础。
[Key word]
[Abstract]
Based on impedance flow cytometer, water culture and simulated high-temperature stress by PCR, the rapid determination system of pollen viability (PV) was established and PV of 227 maize inbred lines were determined at room temperature (25℃) and simulated high-temperature(40℃/30min、50min、70min), which were used in estimation of comprehensive pollen viability (CPV) by BLUE, and correlation analysis with yield of ear in block. At room temperature, variants of PV are abundant and follow normal distribution in maize inbred population. With simulated high-temperature stress, PV exhibited different degree of decline and correlation coefficients with yield of ear was also decreased, which indicated the tolerance of PV to simulated high-temperature stress is significant difference in maize inbred population. A total of 15 maize inbred lines with heat-resistance was obtained by CPV, present high resistance to simulated high temperature. Our research lay the foundation for rapid, quantitative estimation of pollen viability in this paper.
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[基金项目]
国家重点研发计划(2017YFD0101201),河南省农业科学院自主创新项目(2020ZC05)