为明确甘肃省玉米根腐病致病镰孢菌种类，2017年至2019年在甘肃省不同地区采集玉米植株进行组织分离，采用形态学和分子生物学鉴定方法对分离物进行鉴定，并按照柯赫氏法则对其致病性进行测定。结果表明：引起甘肃省玉米根腐病的致病镰孢菌为厚垣镰孢Fusarium chlamydosporum、禾谷镰孢Fusarium graminearum、尖镰孢Fusarium oxysporum、层出镰孢Fusarium proliferatum、茄镰孢Fusarium solani、三线镰孢Fusarium tricinctum、拟轮枝镰孢Fusarium verticillioides和产黄色镰孢Fusarium thapsinum，其中尖镰孢和拟轮枝镰孢为优势镰孢菌，分离频率分别为22.5 %和20 %。通过致病性测定发现所有分离的镰孢菌都会引起玉米根系不同程度的变褐，导致根系生长缓慢，茎基部溢缩和腐烂，近地部叶片黄化，叶边缘干枯等症状。

In order to identify the species of pathogenic Fusarium.sp maize root rott in Gansu province,From 2017 to 2019, collected the maize plants in different regions of Gansu Province for tissue separation.The isolates were identified by morphological identification and molecular biology identification, and their pathogenicity was determined according to Koch's law. The results showed that F. chlamydosporum, F. graminearum,F. oxysporum,F. proliferatum,F. solani,F. tricinctum,F. verticillioides, F. thapsinum caused maize root rot in Gansu province. Among them, F. verticillioides and F. oxysporum were the dominant pathogenic bacterium,the separation frequencies were 20% and 22.5%. Through the virulence test, it was found that all Fusarium sp. could cause the varying degrees browning of maize root. Lead the Root hair reduction, stem base overflow and rot, near the ground leaf yellowing, leaf edge dry and other typical symptoms.

S435.131

甘肃省科技计划(重点研发)项目(18YF1NA011)、甘肃省玉米产业技术体系项目(GARS-02-03)、甘肃省陇原青年人才创新项目（个人）。