YUCCA基因编码生长素合成相关酶,在植物的生长发育过程中发挥重要作用。本研究利用Genevestigator软件分析获得玉米YUCCA(ZmYUC)基因的转录表达谱,并通过PlantCare软件分析ZmYUC基因启动子区的顺式作用元件,获得其在逆境胁迫方面的顺式作用元件,然后利用实时定量PCR方法对ZmYUC基因在冷(4℃)、热(40℃)、盐(250mM的NaCl)和干旱(15%的PEG6000)处理下的表达量变化进行检测,分析ZmYUC基因在不同组织器官及不同逆境胁迫处理下的表达模式,来揭示YUC基因在玉米生长发育及逆境胁迫方面的作用,为全面解析YUC基因在玉米中的生物学功能提供有用信息。

YUCCA genes, encoding enzymes associated with auxin biosynthesis, play essential roles in growth and development of plants by contributing to the local pool of free auxins. The transcriptional profile of maize YUCCA (ZmYUC) gene was analyzed by the Genevestigator software and cis-acting elements of ZmYUC promoter region were analyzed by PlantCare software to obtain cis-acting elements in stress. Then, real-time quantitative PCR was used to detect the expression changes of ZmYUC gene under cold (4℃), heat (40℃), salt (250mM NaCl) and drought (15% PEG6000), and analyze the expression patterns of ZmYUC gene under different tissues and organs and different stress treatments. To reveal the role of YUC gene in maize growth and development and stress, and provide useful information for comprehensive analysis of the biological function of YUC gene in maize.