[关键词]
[摘要]
以耐盐自交系郑58和盐敏感自交系昌7-2为材料,分析供试材料在200 mmol/L NaCl溶液胁迫下0h和5h的转录组数据。与对照0h相比较,在郑58和昌7-2分别鉴定出390和421个上调、390和421个下调差异基因。对抗感材料间共同上调和郑58特有上调DEGs进行GO富集、REVIGO分析和KEGG富集分析以及重点通路分析结果如下:郑58和昌7-2间共同上调DEGs显著富集的条目包括对活性氧代谢过程的调节、内质网应激反应等GO条目,郑58特有上调显著富集含氧化合物的反应、对化学物质的反应等GO条目。共同上调DEGs仅在共单萜生物合成的代谢通路上显著富集,郑58特有上调DEGs在植物激素信号转导、苯丙烷生物合成等6个通路上显著富集,在植物激素信号转导、苯丙烷生物合成两个通路上分别有9和7个相关基因,耐性材料郑58上调的转录因子在MYB和NAC转录因子家族分布数量较多。与植物激素信号转导、苯丙烷生物合成通路相关基因,MYB和NAC转录因子家族基因可能与玉米耐盐性密切相关。研究结果将为今后研究玉米耐盐机理提供了更多的证据,也为培育耐盐品种培育提供材料基础。
[Key word]
[Abstract]
The salt-tolerant inbred line Zheng 58 and the salt-sensitive inbred line Chang 7-2 were used as materials to analyze the transcriptome data of the tested materials under 200 mmol/L NaCl solution stress for 0 h and 5 h. Compared with control 0 h, 390 and 421 up-regulated and 390 and 421 down-regulated differential genes were identified in Zheng 58 and Chang 7-2, respectively. The results of GO enrichment, REVIGO analysis, KEGG enrichment analysis and key pathway analysis of DEGs that are commonly up-regulated between the anti-sense materials and that are specific to Zheng 58 are as follows: The significantly enriched items of DEGs that are jointly up-regulated between Zheng 58 and Chang 7-2 include pairs of active The regulation of oxygen metabolism process, endoplasmic reticulum stress response and other GO items, Zheng 58-specific up-regulated GO items such as the response to oxygenated compounds and the response to chemical substances were significantly up-regulated. The co-up-regulated DEGs were only significantly enriched in the metabolic pathway of co-monoterpenoid biosynthesis. Zheng 58-specific up-regulated DEGs were significantly enriched in six pathways including plant hormone signal transduction and phenylpropane biosynthesis. There were 9 and 7 related genes in the two pathways of phenylpropane biosynthesis, respectively. The transcription factors up-regulated in the tolerant material Zheng 58 were distributed more in the MYB and NAC transcription factor families. Genes related to plant hormone signal transduction, phenylpropane biosynthesis pathway, MYB and NAC transcription factor family genes may be closely related to maize salt tolerance. The research results will provide more evidence for future research on the mechanism of maize salt tolerance, and also provide a material basis for breeding salt-tolerant varieties.
[中图分类号]
[基金项目]
吉林省农业科技创新工程人才(CXGC2021RCY035)、吉林省科技厅重点研发项目(20200402024NC)。