葡萄糖-6-磷酸脱氢酶(Glucose-6-phosphate dehydrogenase，G6PDH)作为磷酸戊糖途径的核心催化酶，通过调控烟酰胺腺嘌呤二核苷酸磷酸(NADPH)的生物合成，在植物氧化应激响应与细胞氧化还原稳态维持中发挥关键作用。本研究系统鉴定获得8个玉米G6PDH基因家族成员，并解析其基因结构、保守基序及蛋白理化特性。基于系统进化分析将拟南芥(十字花科)及玉米和水稻(两种重要禾本科作物)的19个G6PDH基因划分为5组，揭示各组家族成员在基序组成和基因结构上具有显著保守性。经公共数据表达量分析及定量实时PCR(qRT-PCR)验证，低温胁迫下，ZmG6PDH2、ZmG6PDH3、ZmG6PDH5和ZmG6PDH7基因表达量呈显著响应诱导模式。

Glucose-6-phosphate dehydrogenase(G6PDH), as the core catalytic enzyme of the pentose phosphate pathway, plays a crucial role in the oxidative stress response of plants and the maintenance of cellular redox homeostasis by regulating the biosynthesis of nicotinamide adenine dinucleotide phosphate(NADPH). In this study, eight members of the G6PDH gene family were systematically identified in maize, and their gene structures, conserved motifs, and physicochemical properties of the proteins were analyzed. Based on phylogenetic analysis, 19 G6PDH genes from Arabidopsis thaliana(Brassicaceae), maize and rice(two important gramineous crops) were classified into five subfamilies, revealing that the members of each subfamily showed significant conservation in motif composition and gene structure. The expression levels of ZmG6PDH2, ZmG6PDH3, ZmG6PDH5 and ZmG6PDH7 exhibited a significant response induction pattern under low-temperature stress, which were verified by public data expression analysis and quantitative real-time PCR(qRT-PCR).

S513.035.3

吉林省玉米科技创新中心中央引导地方科技发展资金项目(YDZJ202302CXJD037)