为建立玉米南方锈病高通量离体叶片接种鉴定技术，研究玉米离体叶片持绿剂、接种浓度、培养最佳温湿度对人工接种鉴定效果的影响。结果表明：20mg/L 的6-BA和改良霍格兰营养液稀释15倍液按4:1比例混合后对玉米离体叶片持绿效果最佳，以菌液浓度1×105个/mL，培养温度25℃，相对湿度85%-90%为最佳接种鉴定体系。应用该接种鉴定方法评价了农家种对南方锈病的抗性，与田间鉴定结果较为一致。此方法克服了南方锈病田间接种的弊端，为玉米南方锈病的抗性资源发掘、筛选评价及品种选育提供新的技术参考。

To establish a high-throughput in vitro leaf inoculation identification technology for Southern Rust, the effects of green holding agent in vitro maize leaves, inoculation concentration, and the optimal temperature and humidity for cultivation on the artificial inoculation identification effect were studied.The results showed that a mixture of 20mg/L 6-BA and Hoglan nutrient solution diluted 15 times in a ratio of 4:1 had the best greenness retention effect on isolated corn leaves. The optimal inoculation and identification system was based on a concentration of 1×105 cells /mL, a culture temperature of 25℃, and a relative humidity of 85-90%.The resistance of rural varieties to southern rust was evaluated by applying this inoculation identification method, which was relatively consistent with the field identification results.This method overcomes the drawbacks of field inoculation for southern rust disease and provides a new technical reference for the exploration, screening and evaluation of resistance resources and variety breeding of southern rust disease in corn.

河北玉米现代种业科技创新团队（21326319D）；国家现代农业产业技术体系(CARS-02)