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[摘要]
针对农田土壤中多种除草剂复合污染的修复难题,从实验室前期保藏的阿特拉津降解菌中筛选出了一株兼具氟磺胺草醚降解能力的菌株LC-5。该菌株在48 h对100 mg/L氟磺胺草醚的降解率可达62.77%,经生理生化及16S rRNA基因序列分析,鉴定该菌株为节杆菌(Arthrobacter)。菌株LC-5不仅展现出高效降解性能,还具备溶磷、解钾、固氮、产IAA能力,且能耐受pH 9 的碱性环境及 5% NaCl 的盐胁迫,盆栽实验证实施用LC-5菌液可有效缓解土壤中氟磺胺草醚残留对玉米的药害症状,玉米叶片的叶绿素含量、抗氧化酶活性及植株的抗逆性显著提升,丙二醛积累量降低;此外,土壤酶活性也得到激活。高效液相色谱测定结果表明,经LC-5菌液处理后土壤中未检测到氟磺胺草醚残留,综合来看,菌株LC-5具有良好应用前景。
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[Abstract]
To address the challenge of multiple herbicide co-contamination in farmland soil, a strain LC-5 with the ability to degrade fomesafen was screened from the atrazine-degrading bacteria preserved in the laboratory. The degradation rate of fomesafen at a concentration of 100 mg/L by strain LC-5 reached 62.77% within 48 hours. Through physiological and biochemical tests and 16S rRNA gene sequence analysis, the strain was identified as Arthrobacter. Strain LC-5 not only demonstrated high degradation performance but also possessed the abilities of phosphorus solubilization, potassium release, nitrogen fixation, and IAA production. Moreover, it could tolerate an alkaline environment with pH 9 and salt stress of 5% NaCl. Pot experiments showed that the application of LC-5 bacterial liquid could effectively alleviate the phytotoxic symptoms of fomesafen residues in soil on corn. The chlorophyll content in corn leaves, the activity of antioxidant enzymes, and the stress resistance of plants were significantly enhanced, while the accumulation of malondialdehyde was reduced. In addition, soil enzyme activity was also activated. The results of high-performance liquid chromatography indicated that no fomesafen residues were detected in the soil after treatment with LC-5 bacterial liquid. Overall, strain LC-5 shows promising application prospects.
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