探究干旱胁迫下接种丛枝菌根真菌（AMF）明根孢囊霉JX1114、植物根际促生菌（PGPR）解淀粉芽孢杆菌SN16-1及联合接种处理对玉米光合生理特性和产量的影响。以玉米‘沈农T120’种子为试验材料，采用室内盆栽实验，设正常供水（80%田间持水量）、中度干旱（50%田间持水量）、重度干旱（30%田间持水量）条件下分别接种AMF、PGPR、AMF+PGPR联合接种及不接种处理（CK）对玉米叶绿素含量、气体交换参数、营养元素吸收、活性氧代谢、内源激素含量以及单株产量的影响。结果表明:1）正常供水、中度干旱、重度干旱下接种PGPR均可以增加AMF菌根侵染率，接种AMF可以增加PGPR定殖数量，二者具协同作用，重度干旱胁迫下AMF+PGPR联合接种处理的AMF侵染率较AMF处理提高34.6%，PGPR定殖数量较PGPR处理提高31.3%；2）重度干旱胁迫下，与CK相比，AMF+PGPR联合接种的玉米叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量增加14.4%、95.5%、22.2%和42.2%，净光合速率、气孔导度、蒸腾速率较CK分别增加28.9%、23.9%和42.8%，胞间CO2浓度降低8.6%；3）与正常供水相比，中度干旱、重度干旱胁迫下玉米根系和茎内氮、磷、钾含量总体表现为下降趋势，且表现为茎＞根系，重度干旱下AMF+PGPR联合接种的玉米根系氮、磷和钾含量较CK分别增加5.2%、18.5%和19.0%，AMF+PGPR联合接种的玉米茎内氮、磷和钾含量较CK分别增加34.9%、6.8%和13.9%；4）重度干旱胁迫下，玉米叶中超氧化物歧化酶（SOD）、过氧化物酶（POD）活性下降，丙二醛（MDA）和过氧化氢（H2O2）含量增加，AMF+PGPR联合接种的SOD、POD活性增加26.9%和35.4%，MDA和H2O2含量下降26.3%和28.6%；5）重度干旱胁迫下，AMF+PGPR联合接种还能够增加生长素（IAA）和脱落酸（ABA）含量，IAA、ABA含量较CK增加9.0%和10.3%，AMF+PGPR联合接种的玉米百粒质量和单株产量较CK增加3.2%和26.8%。结论认为，对于干旱严重制约作物生产的地区而言，AMF+PGPR联合接种为提升玉米产量提供了更具前景的田间管理策略。

Exploring the effects of inoculation with arbuscular mycorrhizal fungus (AMF) Rhizophagus clarus JX1114, plant growth-promoting rhizobacteria(PGPR) Bacillus amyloliquefaciens SN16-1, and combined inoculation treatment on the photosynthetic physiological characteristics and yield of maize under drought stress. Using corn "Shennong T120" seeds as experimental materials, indoor pot experiments were conducted to investigate the effects of AMF, PGPR, AMF+PGPR combined inoculation, and no inoculation treatment (CK) on maize chlorophyll content, gas exchange parameters, nutrient absorption, reactive oxygen species metabolism, endogenous hormone content, and individual plant yield under normal water supply (80% field capacity), moderate drought (50% field capacity), and severe drought (30% field capacity) conditions. The results showed that: 1) Under normal water supply, moderate drought, and severe drought, inoculation with PGPR could promote AMF mycorrhizal infection rate, and AMF could increase PGPR colonization number, the two had a synergistic effect. Under severe drought stress, the AMF+PGPR combined inoculation treatment increased AMF infection rate by 34.6% and PGPR colonization rate by 31.3% compared to the AMF treatment; 2) Under severe drought stress, compared with CK, the maize inoculated with AMF+PGPR showed an increase of 14.4%, 95.5%, 22.2%, and 42.2% in chlorophyll a, chlorophyll b, total chlorophyll, and carotenoid content. The net photosynthetic rate, stomatal conductance, and transpiration rate increased by 28.9%, 23.9%, and 42.8%, respectively, while the intercellular CO2 concentration decreased by 8.6%; 3) Compared with normal water supply, the overall nitrogen, phosphorus, and potassium content in maize roots and stems under moderate and severe drought stress showed a downward trend, with stems>roots. Under severe drought, the nitrogen, phosphorus, and potassium content in maize roots inoculated with AMF+PGPR increased by 5.2%, 18.5%, and 19.0%, respectively, compared to CK. The nitrogen, phosphorus, and potassium content in maize stems inoculated with AMF+PGPR increased by 34.9%, 6.8%, and 13.9%, respectively, compared to CK; 4) Under severe drought stress, the activities of superoxide dismutase (SOD) and peroxidase (POD) in maize leaves decreased, while the contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2) increased. The activities of SOD and POD increased by 26.9% and 35.4%, while the contents of MDA and H2O2 decreased by 26.3% and 28.6% when inoculated with AMF+PGPR; 5) Under severe drought stress, the combined inoculation of AMF and PGPR can also increase the content of indole-3-acetic acid (IAA) and abscisic acid (ABA), with IAA and ABA content increasing by 9.0% and 10.3% respectively compared to CK. The 100 grains weight and single plant yield of maize inoculated with AMF and PGPR increased by 3.2% and 26.8% respectively compared to CK. The conclusion is that for areas severely constrained by drought, the combined inoculation of AMF and PGPR provides a more promising field management strategy for increasing maize yield.

辽宁省科技计划联合计划项目-面上项目(2024-MSLH-178)；辽东学院博士科研启动资金项目(2022BS008)；辽东学院技术开发(委托)项目(202521060500109)。