以100份CIMMYT引进的优良热带玉米种质为材料，齐319（抗病）和Ye478（感病）为对照，于2024 - 2025年在云南进行田间抗性表型鉴定。从28个分布于已知抗病QTL区的候选SSR标记中筛选多态性标记，并对51份抗性稳定的种质进行聚类分析。田间鉴定筛选出1份高抗（CML172）、10份抗病和24份中抗的稳定种质。最终从候选标记中筛选出8个SSR标记（umc1194，umc1784，bnlg1523，M13，umc2354，umc1515，umc2560，umc1042）构成核心标记集，其中M13、umc2560和umc1515为高多态性标记。基于此标记集的聚类分析将53份材料分为5个类群，抗病种质（CML172、齐319）与感病种质被有效分离。一致性分析显示，SSR标记聚类与表型抗性分类高度一致（Kappa = 0.77，P < 0.0001）。该核心标记集能快速、准确鉴别热带玉米种质对灰斑病的抗性。

Using 100 elite tropical maize germplasms introduced from CIMMYT as materials, with Qi319 (resistant) and Ye478 (susceptible) as controls, field resistance phenotyping was conducted in severe gray leaf spot areas of Yunnan Province from 2024 to 2025. Polymorphic markers were screened from 28 candidate SSR markers located within known resistance QTL regions, and cluster analysis was performed on 51 germplasms with stable resistance. Field identification selected 1 highly resistant (CML172), 10 resistant, and 24 moderately resistant stable germplasms. Finally, 8 SSR markers (umc1194, umc1784, bnlg1523, M13, umc2354, umc1515, umc2560, umc1042) were screened from the candidates to form a core marker set, among which M13, umc2560, and umc1515 were highly polymorphic. Cluster analysis based on this marker set divided the 53 materials into 5 groups, effectively separating resistant germplasms (CML172, Qi319) from susceptible ones. Consistency analysis showed a high agreement between SSR marker clustering and phenotypic resistance classification (Kappa = 0.77, P < 0.0001). This core marker set can rapidly and accurately identify the resistance of tropical maize germplasms to gray leaf spot.

吉林省科技发展计划项目(20240601076RC)