成功克隆玉米硝酸盐转运蛋白基因Zm00001d054057(ZmNRT2.1)，通过生物信息学分析其基本特性。ZmNRT2.1的编码区(CDS)全长为1 575 bp，编码524个氨基酸，其蛋白质的相对分子质量为56 674.73 Da，理论等电点为8.20，具有11个跨膜结构域，并含有硝酸盐跨膜转运体的保守结构域。ZmNRT2.1的启动子区域含有多种与激素和光响应相关的顺式作用元件。系统进化树分析显示，ZmNRT2.1和水稻中OsNRT2.2具有较高的亲缘关系。低氮胁迫条件下的表达模式分析表明，ZmNRT2.1在苗期根和叶中的表达呈现先上调后趋于稳定的趋势，在成熟期的不同组织中表达量相对较低。亚细胞定位显示，ZmNRT2.1蛋白定位于细胞膜。

In this study, the maize nitrate transporter protein gene Zm00001d054057(ZmNRT2.1) was successfully cloned. The fundamental characteristics of the protein were examined through the utilisation of bioinformatics techniques. The coding region of the ZmNRT2.1 gene is 1 575 base pairs in length and encodes 524 amino acids. The relative molecular mass of the protein is 56 674.73 Da, and the theoretical isoelectric point is 8.20. The protein has 11 transmembrane structural domains and contains conserved structural domains of nitrate transmembrane transporters. The promoter region of the ZmNRT2.1 gene contains a variety of cis-acting elements related to hormone response, anaerobic induction response and light response. The phylogenetic tree analysis demonstrated a high degree of relatedness between ZmNRT2.1 and OsNRT2.2 in rice. An analysis of the expression pattern under low nitrogen stress conditions revealed that the expression of the ZmNRT2.1 gene in roots and leaves at the seedling stage exhibited a tendency towards up-regulation, followed by stabilisation. Conversely, the expression in different tissues at the maturity stage was relatively low. Subcellular localisation experiments revealed that the ZmNRT2.1 protein is localised to the cell membrane. In conclusion, these results provide a theoretical and experimental basis for a deeper understanding of the crucial role of ZmNRT2.1 in maize nitrogen utilization.

S513.035.3

国家现代农业产业技术体系项目;四川省突破性玉米及高粱育种材料和方法创新及品种选育(育种攻关项目)(2021YFYZ0017)