为开发新型耐除草剂玉米转化体，提高田间杂草防控效率，通过农杆菌介导玉米幼胚转化法，将含Bar、dicX和EPSPS基因的载体pWEBA-dicX转入玉米自交系CAL50幼胚中并获得转基因玉米。利用PCR和胶体金试纸条筛选阳性转基因株系，通过叶片涂抹法筛选出23个耐受1倍田间推荐中剂量浓度的草铵膦和麦草畏转基因株系，进一步针对Bar和EPSPS基因在转录和翻译水平的分子特征进行评价，为转基因耐除草剂玉米的研发提供材料和技术支撑。

In order to develop a new transgenic herbicide-tolerant maize and improve the control efficiency of weeds in the field, the overexpression vector pWEBA-dicX containing the Bar, dicX and EPSPS genes was introduced into young embryos of maize inbred line CAL50, yielding transgenic herbicide tolerant maize. Positive transgenic lines were identified through PCR and colloidal gold test strip, and 23 transgenic lines exhibiting resistance to the recommended medium dose concentration of Glufosinate ammonium and Dicamba were selected using the leaf coating method. Furthermore, molecular characterization of the Bar and EPSPS genes at both the transcriptional and translational levels was conducted to provide essential material and technical support for developing transgenic herbicide-tolerant maize.

S513.035.3

中国农业科学院科技创新工程项目(CAASZDRW202401)