Archive > Volume 28 Issue 5 > 2020,28(5):32-38. DOI:10.13597/j.cnki.maize.science.20200506 Prev Next
Cloning and Expression Analysis of Heat Shock Protein Gene ZmHSP70-12 in Maize
- Article
- Figures
- Metrics
- Preview PDF
- Reference
- Related
- Cited by
- Materials
Abstract:
Heat shock protein 70(HSP70s) are found extensively in prokaryotic and eukaryotic and one of the most conserved molecular chaperones. In this study, a member of the HSP70 family was isolated from maize. Its fulllength coding sequence was 1 992 bp, and its open reading frame is 2 352 bp. The encoded protein with molecular weight is about 75.0 kD. Through protein structure prediction and homologous alignment analysis, the gene encodes a protein containing ATPase site and HSP70 conserved domain. It is highly similar to Arabidopsis thaliana AtHSP70-12 sequence, so it is named ZmHSP70-12. Subcellular localization analysis showed that the ZmHSP70-12 protein was expressed in the endoplasmic reticulum. Real-time quantitative PCR analysis revealed ZmHSP70-12 has an obvious response to abiotic stress, such as high temperature and drought. Therefore, it speculates that ZmHSP70-12 is a stress-related gene in maize stress.
Keywords:
Project Supported:
Clc Number:
S513.032
