Archive > Volume 28 Issue 5 > 2020,28(5):47-52. DOI:10.13597/j.cnki.maize.science.20200508 Prev Next
Transgenic Corn Research by Emery-assisted Agrobacterium-Mediated Transformation
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Abstract:
The ultrasonication-assisted Agrobacterium treatment method was modified with the addition of emery to increase the treatment intensity to the apical meristem cells of germinating seeds. Ninety three plants from 800 control seeds were sprayed three times with glyphosate solution, and 27 of them survived, their leaves were taken with a PCR electrophoresis test and 23 shown positive, which suggested that epsps gene was introduced into the corn cultivar, and the transformation rate was 29%. Finally, which has been shown that the method has the advantages of circumventing the tedious tissue culture procedures and being easy to apply.
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Clc Number:
S513.035.3
