Dynamic expression patterns of corresponding enzymes of Histone acetylation modification in Maize under nitrogen deficiency
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Abstract:
Histone acetylation modification as key epigenetic factors in regulating the expression of genes in multiple aspects of plant growth, development and plant response to abiotic or biotic stresses. A better understanding of how histone acetylation modification fine-tunes its physiological and developmental processes to fluctuating N concentrations in the soil is needed to improve N use efficiency in maize production. N limitation generally inhibited ear growth. The ear was 16.2%-19.7% shorter from 1 to 2 weeks after silking (OAS; TAS) under N limitation compared to that with suf?cient N supply. During the same time, the dry weight (DW) of the N de?cient ear was 35.6%-44.4% less than that of the N suf?cient ear. The N concentration abruptly dropped in the maize ear at TAS, with an approximately 13.3% decrease. qRT-PCR amplification were conducted to analyze maize seedling stage and key period of maize ear developmnet, how nitrogen deficiency affects the expression level of histone acetylases (GCN5 and HAT-B) and deacetylases (HD1b, HD2, HDA101, HDA102, HDA106 and HDA108). During the seedling stage, all HTAs and HDACs were dierentially regulated in response to low nitrogen treatment for 8 days, except HAT-B, which did not respond to nitrogen deficiency. the expression of HDA102 and HDA108 decreased in response to low nitrogen treatment for 12 h relative to the control, while the expression of HAT-B, HDA101, HD1b and HD2 increased after 36 h of nitrogen deficiency treatment. In addition, the expression of HD1b was also significantly up-regulated after 4 d of nitrogen deficiency. After low nitrogen treatment for 2 days, the expression of HDA106 and HDA108 were down-regulated. At silking stage, except HAT-B, HD1b and HD2 genes, all genes had a higher expression levels under nitrogen deficiency. At one week before silking, the expression of HDA101 and HDA106 were significantly up-regulated due to nitrogen deficiency. However, the expression of HD1b and HD2 were significantly down-regulated at two week after silking. Instead, the expression of HDA101 was up-regulated in nitrogen-deficient ear at two week after silking. In addition, the expression of HDA106 decreased in response to low nitrogen at one week after silking. The regular expression of histone acetylases and deacetylases under nitrogen deficiency indicated that an important role of histone acetylation modification in maize’s adaptation to nitrogen deficiency stresse during growth and development.