QTL Mapping and Candidate Gene Analysis about Ear-Related Traits in Maize Based on High Density SNP Markers
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Abstract:
T32 and Qi319 were used as parents to construct 118 F2:3 families. Ear related traits of different families were evaluated, and genotype of each plants were identified by using high-density SNP markers. QTL mapping was conducted for 5 Ear-Related traits (ear length, ear diameter, ear row number, kernel number per row, ear barren tip) by using composite interval mapping method in IciMapping4.2 software. A total of 16 QTLs were detected, wherein 3, 2, 4, 2 and 5 QTLs were detected to be related with ear length, ear diameter, ear row number, kernel number per row and ear barren tip, respectively. A single QTL could explain phenotypic variation of 2.92% ~ 13.53%. Based on the public database, 5 suspected key candidate genes related with spike related traits were screened by using bioinformatics analysis, which including genes of Zm00001d031906, Zm00001d02721, Zm00001d002762, Zm00001d002768. These results would provided much more genetic locus for the genetic improvement of ear traits in corn, and some reliable candidate regions for the exploration of candidate genes' function.
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