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玉米泛素活化酶基因家族鉴定及表达模式分析
Identification and Expression Analysis of the Ubiquitin Activating Enzyme Gene Family in Maize
投稿时间:2017-05-24  
中文关键词:玉米  泛素活化酶  生物信息学  基因表达
英文关键词:Maize  Ubiquitin activating enzyme(UBA) gene  Phylogeny analysis  Gene expression
基金项目:中国热带农业科学院基本科研业务费专项(1630062015003,1630062017006,1630062016013)
作者单位E-mail
赵秋芳 中国热带农业科学院南亚热带作物研究所/农业部热带果树生物学重点实验室, 广东 湛江 524091  
贾利强 中国热带农业科学院南亚热带作物研究所/农业部热带果树生物学重点实验室, 广东 湛江 524091 lqjia@mail.zaas.ac.cn 
马海洋 中国热带农业科学院南亚热带作物研究所/农业部热带果树生物学重点实验室, 广东 湛江 524091  
陈曙 中国热带农业科学院南亚热带作物研究所/农业部热带果树生物学重点实验室, 广东 湛江 524091  
陈宏良 中国热带农业科学院南亚热带作物研究所/农业部热带果树生物学重点实验室, 广东 湛江 524091  
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中文摘要:
      通过生物信息学分析,从B73玉米全基因组中鉴定出4个泛素活化酶基因,分别命名为ZmUBA1~ZmUBA4。4个ZmUBA基因编码氨基酸数目在1 030~1 056 aa,编码蛋白分子量在114.63~117.39 kD,等电点在5.18~5.80,且均含有5个内含子。蛋白二级结构预测,4个基因编码的蛋白主要以α-螺旋和不规则卷曲为主,亚细胞定位预测4个基因均定位于细胞核中。荧光定量PCR结果表明,ZmUBA1基因在根、茎、叶、雄穗和雌穗中呈现组成性表达,ZmUBA2ZmUBA4基因在雄穗表达量最高,ZmUBA3在叶片表达量最高,呈现组织特异性表达。ZmUBA3在盐和低温胁迫上调表达,ZmUBA4在盐胁迫时下调表达,说明ZmUBA3基因可能参与玉米低温和盐胁迫的应答,ZmUBA4可能参与玉米盐胁迫应答。
英文摘要:
      Four ubiquitin activating enzyme(UBA) genes which designated as ZmUBA1-4 were identified from the whole genome of B73 maize by using bioinformatics method. The polypeptide lengths of the ZmUBA genes ranged from 1 030 aa to 1 056 aa, and their predicted molecular weights ranged from 114.63 kD to 117.39 kD. The predicted pI had a range of 5.18 to 5.8, and all of the ZmUBA genes contained 5 introns. The predicted secondary structure suggested that the main structure of four proteins were alpha helix and random coil. The subcellular localization analysis showed that all of the ZmUBA proteins were located in the nucleus. Real-time qPCR assay showed that ZmUBA1 gene was co-expressed in roots, stems, leaves, male spikes and female ears, the highest expression level of ZmUBA2 and ZmUBA4 were occured in the male spikes and ZmUBA3 occured in leaves, and three genes showed tissue specific expression. The exression of ZmUBA3 was up-regulated under salt and low temperature stresses, and ZmUBA4 gene was down-regulated under salt stress, These results indicated that ZmUBA3 may be involved into plant response to salt and low temperature stresses, and ZmUBA4 only involved into plant response to salt stress.
赵秋芳,贾利强,马海洋,陈曙,陈宏良.玉米泛素活化酶基因家族鉴定及表达模式分析[J].玉米科学,2018,26(1):8~14,19
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