[关键词]
[摘要]
采用多个测验种逐代跟踪测定诱导率的方法,利用俄罗斯引进的单倍体诱导系资源,经过5年7代选育出辽诱系列玉米单倍体诱导系辽诱1号、辽诱2号、辽诱3号和辽诱4号。对22个品种的诱导效果试验表明,辽诱1号、辽诱2号、辽诱3号和辽诱4号的综合诱导率分别为6.85%、7.53%、6.66%和6.03%,比对照高诱五号分别高出0.6倍、0.8倍、0.6倍和0.4倍。诱导基因检测结果表明,辽诱系列诱导系具有诱导率高、花粉量大、抗病、抗倒、易繁殖等优良特性,且存在和高诱五号共同的诱导基因GRMZM2G471240,是开展玉米单倍体育种的理想诱导系。
[Key word]
[Abstract]
A batch of haploid inducer resources was introduced from Russia in 2013 and used for breeding. LAAS-1, LAAS-2, LAAS-3 and LAAS-4 were bred by using multi-test materials with high frequency inducing rate (IR) through 7 generations between 5 years. The results of induction effect test for 22 entries showed that the IR of LAAS-1, LAAS-2, LAAS-3 and LAAS-4 were 6.85%(0.6 times higher than CK), 7.53%(0.8 times higher than CK), 6.66%(0.6 times higher than CK) and 6.03%(0.4 times higher than CK), respectively. The results of induced gene test indicated that the LAAS-set haploid inducers and CAU-5 shared the same induced gene GRMZM2G471240. LAAS-set haploid inducers are ideal inducers for haploid breeding of maize at present in good characteristics such as high inducing rate, abundant pollen quantity, resistance to blight and lodging, easy reproduction, etc.
[中图分类号]
[基金项目]
农业部转基因生物新品种培育重大专项(2016ZX08003003-008)、辽宁省农业领域青年科技创新人才培养计划项目(2015024)、辽宁省科技厅"中俄(辽宁)创新创业基地项目