为了明确山西省玉米大斑病菌生理小种与交配型的结构组成,利用Ht单基因鉴别寄主对2022年采自山西省11个市30个市/区/县的87株玉米大斑病菌进行生理小种鉴定,并采用交配型鉴定特异性引物对供试菌株进行交配型测定。结果表明:山西玉米大斑病菌生理小种结构组成十分复杂,87株病菌中共发现0、1、N、13、23、1N、2N、3N、123、12N、13N、23N、123N等13个生理小种,123N号以22.99%分布频率成为优势生理小种,N号以16.09%分布频率成为次优势生理小种。供试菌株对Ht1、Ht2、Ht3和HtN的毒性频率分别为56.32%、47.13%、49.43%、86.21%。交配型测定结果显示,87株分离株中包括3种交配型,即A、a和Aa交配型,其比例分别为43.68%、51.72%和4.60%。

In order to clarify the structure of physiological race and mating type of Setosphaeria turcica in Shanxi Province, the physiological races of 87 isolates of S. turcica collected from 30 cities/districts/ counties in 11 geographical regions of the province in 2022 were identified by using differential hosts containing Ht single resistance gene, and the mating type specific primers were used to determine the mating type of the tested isolates. The results showed that the composition of physiological races of S. turcica in Shanxi was very complex, a total of 13 physiological races were identified in 87 isolates, such as 0, 1, N, 13, 23, 1N, 2N, 3N, 123, 12N, 13N, 23N and 123N, in which race 123N and race N were the dominant and sub-dominant races with the distribution frequencies of 22.99% and 16.09 %, respectively. The toxicity frequency of the tested isolates to Ht1, Ht2, Ht3 and HtN was 56.32%, 47.13%, 49.43% and 86.21% respectively. The results of mating type determination showed that there were three mating types of A, a and Aa in 87 isolates，with the proportion of 43.68%, 51.72% and 4.60% respectively.

忻州市科技计划项目(20220506)；山西省基础研究计划青年科学研究项目(202203021212438)