东北玉米骨干自交系在诱导愈伤组织和植株再生中,基因型差异非常显著.自交系7922最高愈伤诱导率达89.1%,芽苗率为66.4%;而最低的921诱导率仅有18.9%,芽苗率为0.再生途径主要有两种:7922主要以体胚发生途径为主,4122、8902主要以器官发生途径为主.2,4-D是体胚诱导不可缺少的植物生长激素,适当的细胞分裂素能促进诱导4112、8902不定芽的产生.而且,再生途径并不是一成不变的,在一定条件下可以相互转变。

There existed great differences among maize genotypes when immature embryos were cultured fnrplant regeneration. 7922 showed the highest frequencies of callus induction and shoot formation which were 89.1％．and 66.4％respectively While the lowest was found in 921．the induction and regeneration frequencies were 18.9％and O respectively There are two regeneration pathways. 7922 tended to regenerate via somatic embryogenesis，4112，8902 arelikely to regenerate via organogenesis 2,4-D was essential fnr inducing somatic embryoids．and suitable eytokin canpromote adventitious shoot ormation of 4112 and 8902. Furthermore，the regeneration pathways are interchangeableunder different culture conditions.

国家转基因植物中试及产业化基地(吉林)专项资助(JY03-17-1)