[关键词]
[摘要]
分别用不同低浓度玉米小斑病菌C毒素培养滤液处理两种基因型(B37和C103)同核异质系的玉米叶片后再接高浓度毒素培养滤液,观察其病斑大小并测定与抗病相关的关键酶——苯丙氨酸解氨酶(PAL)活性变化。结果表明,对CB37的处理,以1∶50处理效果较好,PAL酶活性最高;对CC103的处理,以1∶60处理效果较好,PAL酶活性最高。在不同基因型、不同细胞质中所需激发子最有效地激活浓度及激活时间不同,确定了低浓度C毒素作为激发子来诱导玉米的获得性抗性具有一定的广泛性。
[Key word]
[Abstract]
The low concentration of Bipolaris maydis race C toxin as an elicitor is extensive and can be repeatable. We detained the same results on a pair of homokaryon B37 and C103 lines. At the same time,we measured the dimension of the leaf spot and determined the changes of PAL activities. The effect of 1 ∶ 50 in different disposal of CB37 is better, but in CC103 1 ∶ 60 is. Accordingly, the right concentration of different breeds is discriminative.
[中图分类号]
[基金项目]
河北省自然科学基金项目(C2006000744)、国际科技合作项目(2006DFB02480)、河北省农林科学院发展研究项目(A03-2-01-14)、河北省科技项目(07297162D)