选用玉米优良自交系郑58的茎尖为受体，研究以玉米茎尖为受体进行农杆菌转化体系的可行性。用农杆菌介导法将耐盐碱基因HAL1转入玉米中，获得70株转化苗，经过300 mg/L的除草剂(Basta)筛选，共获得9株转基因植株。进一步进行 PCR检测，其中6株表现阳性，转化率达8.57%。初步证明外源基因已经整合到玉米基因组中，以玉米茎尖作为受体孢子体的转化系统可用于基因转化。

The HAL1 gene was transferred into the shoot apical point of maize inbred line zheng 58 by Agrobacterium tumefaciens mediated transformation to study the feasibility of this system. Nine transgenic plants were obtained after selecting with 300 mg/L Basta and 6 of them were confirmed positive by PCR amplification. The frequency of transformation reached about 8.57%. The study showed that the foreign gene has integrated into maize genome and the shoot apical point of maize as receipt sporophyte can be used for transformation.

吉林省科技发展计划项目(20076010)