SSR(Simple Sequence Repeat)分子标记技术已经成为玉米常规育种某些性状辅助选择的重要手段，需要对大量个体样本进行标记分析。高效、快速提取植物组织基因组DNA是关键的技术环节。本研究以高油玉米回交世代群体25～30 d单株幼叶为材料，建立以EDTA、CTAB、KCl为主要试剂，结合组织研磨棒液氮研磨为主要步骤的基因组DNA快速提取方法，并探讨了将提取的DNA应用于SSR分子标记辅助选择的可行性。

Some molecular technologies including SSR(Simple Sequence Repeat) have been important methods for maize traditional breeding and the process needs analysis of a large individual samples.So the method for DNA extraction with rapid and efficient is essential.In this study,young leaves per plant of high oil maize backcross generation groups 25 - 30 d as the material,the establishment of EDTA,CTAB,KCl as the main reagent,and tissue grinding rod mill in liquid nitrogen as the main steps of the rapid extraction of genomic DNA,discussed the possibility for DNA which be extracted used in SSR marker-assisted selection.

国家“863”课题 (2006AA100103)、山东省农业科学院创新基金项目(2006YCX004、2006YCX005)