采用碘-碘化钾法、氯化三苯基四氮唑(TTC)法及离体萌发法对花粉活力进行了室内测定，并与大田测定结果作了相关分析。结果表明：碘-碘化钾法不适合于测定玉米花粉活力，氯化三苯基四氮唑(TTC)法及离体萌发法能有效测定玉米花粉活力,TTC法来测定花粉的活力最为简单、准确。

In this paper,three methods were used to detemined the pollen viability of maize,including iodine-iodide kalium method,triphenyl tetrazolium chloride(TTC) method and germination in vitro.The results showed that the method of iodine-iodide kalium could not be able to describe the pollen viability of maize.The methods of TTC and germination in vitro were both fit for detection of pollen viability of maize and the former was more simple and nice.

河北省自然科学基金项目(398079)