[关键词]
[摘要]
采用生物信息学的方法,在玉米全基因组水平鉴定并分析玉米LIM蛋白基因,鉴定出11个ZmLIM基因,分别分布于玉米的8条染色体上。结构域分析表明,多数LIM蛋白包括2个典型的LIM结构域。系统发育分析表明,LIM家族可以分为3个亚家族。假定调控元件分析发现,所有LIM基因启动子都具有大量花粉特异表达元件,相对来说其他组织器官特异表达,激素和逆境调控元件数量很少。EST基因表达数据分析表明,该家族成员具有明显的时空表达特性。
[Key word]
[Abstract]
By bioinformatic analysis, maize LIM genes were isolated and analyzed in those functions in whole-genome scale. A total of 11 maize LIM genes(ZmLIM1 to ZmLIM11) were identified. ZmLIM genes were distributed in all the maize chromosomes except chromosome 7 and 8. Alignment and motif analysis results revealed major maize LIM proteins share two typical conserved domains. Phylogenetic analysis indicated LIM family could be divided into three subfamilies. Putative cis-acting regulatory elements involved in specific tissue or organ, phytohormones and stress, the tissue-specific expression elements, especial pollen-specific element, were observed in the promoters of all ZmLIM genes; other elements distribution presented fragmentarily. Expression data of ESTs suggested maize LIM genes had temporal and spatial expression pattern.
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[基金项目]
“十二五”农村领域国家科技计划项目子课题(2011 BAD16B1103)、大庆市科技计划项目(scyh-2011-73)、黑龙江省寒地作物种质改良与栽培重点实验室开放课题项目(CGIC201208)、黑龙江省垦区科研项目(NK12A- 03-15)、黑龙江八一农垦大学引进人才科研启动计划课题