以先玉335为母本、高频孤雌生殖诱导系G5为父本杂交诱导产生的单倍体为实验材料, 研究秋水仙素浸芽加倍处理玉米单倍体的最佳浓度和时间。结果表明, 秋水仙素浓度为0.7 mg/mL、浸芽8 h处理下的玉米单倍体加倍率最高, 高达14.4%;秋水仙素浓度为0.8 mg/mL、浸芽8 h处理下的单倍体加倍率次之, 为12.1%。以自交系PHB1M、PH4CV、PH6WC、PHGJ4和DH系Z2、Z8为亲本组配的PHB1M×PH4CV、PHB1M×Z2、PH4CV×DZ2、PH6WC×PHGJ4、PH6WC×Z8、PHGJ4×Z8组合为母本, 以G5为父本, 研究玉米DH系对杂交后代加倍率的影响, 结果表明, PH4CV×Z2(26.2%)、PHB1M×Z2(25.8%)、PHGJ4×Z8(23.3%)和PH6WC×Z8(21.3%)的单倍体加倍率显著高于PHB1M×PH4CV(13.5%)和PH6WC×PHGJ4(8.4%), 以DH系为亲本组配选系基础材料可显著提高玉米杂交组合的单倍体加倍率。

Used Xianyu335 as the female parent and the haploid frequency parthenogenesis inducer G5 as the male parent, the optimum concentration and time of maize haploid colchicine soaked bud treatment were studied. The results showed that the doubling rate of maize haploid treated by colchicine soaked bud with concentration of 0.7 mg/mL and under 8h was the highest, up to 14.4%.What was more, the higher was 12.1% with that the concentration of 0.8 mg/mL and under 8h treatment. Six combinations of PHB1M×PH4CV, PHB1M×Z2, PH4CV×DZ2, PH6WC× PHGJ4, PH6WC×Z8, PHGJ4×Z8 that were composited by inbred lines of PHB1M, PH4CV, PH6WC, PHGJ4 and DH lines of Z2 and Z8 as the female parent, also with G5 as male parent to study the doubling effects of DH lines on maize hybrids. The results showed that the haploid doubling rate of PH4CV×Z2(26.2%), PHB1M×Z2 (25.8%), PHGJ4×Z8 (23.3%) and PH6WC× Z8(21.3%) were significantly higher than that of PHB1M×PH4CV (13.5%) and PH6WC×PHGJ4(8.4%). With basic materials of DH lines could significantly improve the doubling rate of maize hybrid combinations.

国家“863”项目(2011AA10A103)、农业部“948”项目(2011-G1-21)